Open AccessFrequent detection of cytomegalovirus and Epstein–Barr virus in cervical secretions from healthy young women
Author(s) -
Berntsson Matilda,
Dubicanac Lejla,
Tunbäck Petra,
Ellström Agneta,
Löwhagen GunBritt,
Bergström Tomas
Publication year - 2013
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1111/aogs.12134
Subject(s) - herpes simplex virus , asymptomatic , cytomegalovirus , virology , virus , herpesviridae , medicine , cervical cancer , cervix , dna , viral shedding , human cytomegalovirus , real time polymerase chain reaction , viral disease , biology , cancer , gene , genetics
Objective To investigate asymptomatic shedding from the uterine cervix of five human herpes viruses: cytomegalovirus ( CMV ), Epstein–Barr virus ( EBV ), herpes simplex virus ( HSV ) type 1 and type 2 and varicella zoster virus ( VZV ), in young women. Design A descriptive study. Setting Sahlgrenska University Hospital. Population Three hundred and five young, healthy Swedish women. Methods Cervical specimens were analyzed for the presence of viral DNA with a quantitative real‐time PCR assay. Main outcome measures Detection of viral DNA . Results Viral DNA was detected in 66 (21.6%) of the cervical samples. The most common findings were CMV DNA, detected in 35 (11.5%), and EBV DNA, found in 32 (10.5%) of the women. HSV‐1 DNA was detected in 5 (1.7%) and HSV‐2 DNA in 4 (1.4%), but VZV DNA was not found. The estimated DNA level for the detected viruses was similar with a mean DNA quantity of 2.6 log genome equivalents (Geq)/mL for CMV (range 1.7–4.3), 2.5 log Geq/mL for EBV (range 1.7–4.7), 2.4 log Geq/mL for HSV‐1 (range 1.7–3.5) and 2.6 log Geq/mL for HSV‐2 (range 1.7–4.1). The simultaneous presence of DNA from two or more herpes viruses was detected in eight specimens. Conclusions Asymptomatic shedding of CMV and EBV from the uterine cervix was found in one‐fifth of young women. In four of the cervical samples; two with EBV , one with CMV , one with HSV ‐2, high amounts of viral DNA (>4 log Geq/mL) were detected suggesting a greater risk of transmitting the virus perinatally or sexually.