Open AccessThe alleviative effects of taurine supplementation on growth, antioxidant enzyme activities, hepatopancreas morphology and mRNA expression of heat shock proteins in freshwater prawn Macrobrachium nipponense (De Haan) exposed to dietary lead stress
Author(s) -
Ding Zhili,
Kong Youqin,
Qi Changle,
Liu Yan,
Zhang Yixiang,
Ye Jinyun
Publication year - 2021
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.13354
Subject(s) - hepatopancreas , malondialdehyde , taurine , biology , catalase , oxidative stress , superoxide dismutase , endocrinology , glutathione peroxidase , medicine , antioxidant , biochemistry , food science , zoology , amino acid
To investigate the potential alleviative effect of taurine supplementation on growth and oxidant stress in freshwater prawn Macrobrachium nipponense (De Haan) exposed to dietary lead, pelleted feeds were prepared with two concentrations of lead (0 and 10 mg/kg diet) and two levels of taurine (0 and 15 g/kg diet). Healthy prawns were randomly distributed between the four treatment groups, with three tanks per group and 50 prawns per tank. Dietary lead decreased weight gain and the activities of superoxide dismutase, catalase and glutathione peroxidase; increased malondialdehyde content; altered hepatopancreas morphology; and decreased the expression of heat shock protein 90 (HSP90; p < .05). A significant interactive effect was found between dietary lead and taurine on the malondialdehyde level, and taurine supplementation significantly decreased the malondialdehyde level under lead stress ( p < .05). No significant effect on growth, hepatopancreas morphology and HSP90 expression was observed with taurine supplementation under lead stress. Overall, dietary lead affected growth, induced oxidative stress and impaired hepatopancreas morphology of the prawns, and dietary taurine significantly alleviated oxidative stress through a decreased malondialdehyde level, whereas the capacity of taurine to alleviate the decreased growth rate, hepatopancreas damage and low HSP90 expression under lead stress was limited.