Open AccessDietary carbohydrate‐to‐lipid ratios modulate juvenile hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus ): Effects on growth, serum biochemistry, intestinal digestion and hepatic metabolism of glucose and lipid
Author(s) -
Chen Guofeng,
Qian Jiahao,
Liu Hongyu,
Tan Beiping,
Dong Xiaohui,
Yang Qihui,
Chi Shuyan,
Zhang Shuang
Publication year - 2021
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.13275
Subject(s) - biology , grouper , lipid metabolism , medicine , endocrinology , lipogenesis , carbohydrate metabolism , hepatic lipase , lipolysis , biochemistry , carbohydrate , pyruvate carboxylase , acetyl coa carboxylase , lipid digestion , gluconeogenesis , digestion (alchemy) , metabolism , enzyme , lipase , adipose tissue , chemistry , chromatography , fishery , fish <actinopterygii>
A 56 day feeding trial was conducted to study effects of different carbohydrate‐to‐lipid (CHO.L −1 ) ratios on growth, serum biochemistry, intestinal digestion and hepatic metabolism of glucose and lipid in juvenile hybrid grouper ( Epinephelus fuscoguttatus × ♂ E . lanceolatus ). Results showed that moderate CHO.L −1 ratio resulted in better growth performance ( p < .05). In serum, glucose, insulin and alanine aminotransferase were enhanced as CHO.L −1 ratios increased, whereas cholesterol was reduced ( p < .05). Increased CHO.L −1 ratios resulted in raised activity of amylase and reduced activity of lipase in gut ( p < .05). Gene expression related to glucose transporters improved ( p < .05). Enzyme activity and gene expression involved in hepatic glycolysis improved in a limited manner, whereas enzyme activity and gene expression related to hepatic gluconeogenesis and lipolysis were inhibited in high CHO.L −1 ratio group. Enzyme activity of hepatic lipogenesis showed an elevated trend, including acetyl‐CoA carboxylase and fatty acid synthase ( p < .05). In conclusion, different dietary CHO.L −1 ratios affected growth, serum biochemistry, intestinal digestion and hepatic metabolism of glucose and lipid in grouper. Moreover, juvenile grouper could utilize diet containing moderate CHO.L −1 ratio.