Open AccessDietary fishmeal levels affect anti‐oxidative ability and metabolomics profile of juvenile Pacific white shrimp, Litopenaeus vannamei
Author(s) -
Xie Shiwei,
Wei Dan,
Chen Shijun,
Zhuang Zhenxiao,
Yin Peng,
Liu Yongjian,
Tian Lixia,
Niu Jin
Publication year - 2020
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.13055
Subject(s) - litopenaeus , shrimp , hepatopancreas , hemolymph , biology , malondialdehyde , superoxide dismutase , alkaline phosphatase , catalase , fish meal , glutathione , acid phosphatase , food science , biochemistry , zoology , antioxidant , ecology , enzyme , fishery , fish <actinopterygii>
Abstract In this manuscript, three iso‐nitrogenous and iso‐lipidic diets containing 50, 150 and 250 g/kg of fishmeal (FM) were fed white shrimp Litopenaeus vannamei for 8 weeks. At the end of feeding, survival and percentage weight gain of shrimp were examined. Hepatopancreas and haemolymph were sampled, activities of superoxide dismutase (SOD) and catalase (CAT), nitric oxide (NO), glutathione (GSH) and malondialdehyde (MDA) levels in hepatopancreas, activities of SOD, CAT, acid phosphatase (ACP) and alkaline phosphatase (AKP), and NO, GSH, MDA in haemolymph were examined. Haemolymph was further subjected to GC‐MS analysis. Results indicated that no significant differences in survival rate and percentage weight gain were observed among three treatments. SOD activity and GSH levels in hepatopancreas, and CAT activity, AKP activity, GSH and MDA levels in haemolymph were significantly lower in shrimp fed 50 g/kg FM diet. GC‐MS analysis of haemolymph indicated that 81 metabolites were significantly altered in the three groups. Furthermore, 8 metabolism pathways were significantly influenced by dietary FM levels. In conclusion, dietary administration of 50 g/kg FM resulted in inhibition of antioxidant ability, and disordered the energy metabolism, one‐carbon metabolism and unsaturated fatty acid metabolism in shrimp.