Open AccessEvaluation of fermented date palm seed meal with Aspergillus oryzae on the growth, digestion capacity and immune response of Nile tilapia ( Oreochromis niloticus )
Author(s) -
Dawood Mahmoud A. O.,
Eweedah Nabil M.,
Khalafalla Malik M.,
Khalid Ahmed
Publication year - 2020
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.13042
Subject(s) - nile tilapia , oreochromis , biology , aspergillus oryzae , food science , fermentation , lysozyme , meal , globulin , protease , digestion (alchemy) , weight gain , immune system , zoology , biochemistry , immunology , body weight , endocrinology , enzyme , chemistry , fish <actinopterygii> , chromatography , fishery
Five diets were prepared to include date palm seed meal (DSM) fermented with Aspergillus oryzae (ASP) at 0, 50, 100, 150 and 200 g/kg diet and fed for Nile tilapia (26.93 ± 0.01 g) for 8 weeks. Fermented DSM significantly affected the final weight, weight gain and specific growth rate in a dose‐dependent manner ( p = .001) with insignificant differences in feed conversion ratio ( p > .05). Dietary fermented DSM significantly increased the lipase, amylase and protease activities ( p = .008, p = .001 and p = .01, respectively) and the Hb, RBCs and WBCs ( p = .001, p = .005, and p = .009, respectively). The intestinal villus length and number of goblet cells were significantly affected by fermented DSM inclusion ( p < .05). Additionally, the total protein, albumin and globulin significantly affected by the inclusion of fermented DSM in a dose‐dependent manner ( p = .04, p = .045, and p = .03, respectively). Furthermore, dietary fermented DSM significantly altered the phagocytic index, phagocytic activity and lysozyme activity ( p = .001, p = .045 and p = .04, respectively). Based on the polynomial regression analysis, the inclusion of fermented DSM at 103.3–164.8 g/kg diet can be used effectively in tilapia diets for improving the growth, digestion activity and immune response.