Open AccessThe antioxidant status, apoptosis, intercellular integrity and immune function of grass carp ( Ctenopharyngodon idella ) head kidney and spleen fed different levels of pyridoxine
Author(s) -
Jiang Wei-Dan,
Zheng Xin,
Feng Lin,
Wu Pei,
Liu Yang,
Jiang Jun,
Kuang Sheng-Yao,
Tang Ling,
Zhou XiaoQiu
Publication year - 2020
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.13022
Subject(s) - grass carp , biology , malondialdehyde , antioxidant , medicine , endocrinology , fas ligand , spleen , pyridoxine , apoptosis , biochemistry , immunology , programmed cell death , fishery , fish <actinopterygii>
This study was carried out to investigate the effects of dietary pyridoxine (PN) on the antioxidant status, apoptosis, intercellular integrity and immune function of head kidney and spleen in young grass carp ( Ctenopharyngodon idella ). Results showed that compared with the optimal PN level, (a) PN deficiency decreased antioxidant enzyme activities and down‐regulated the mRNA levels of antioxidant enzymes, NF‐E2‐related factor 2 (Nrf2), myeloid cell leukaemia‐1 (Mcl‐1) and tight junction proteins, whereas it increased the contents of reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC), and up‐regulated the mRNA levels of cysteinyl aspartic acid‐protease (caspase), Fas ligand (FasL), p38 mitogen‐activated protein kinase (p38MAPK) and myosin light chain kinase (MLCK) in the head kidney and spleen of fish ( p < .05). (b) PN deficiency decreased lysozyme (LZ) and acid phosphatase (ACP) activities, and complement contents, and down‐regulated the mRNA levels of antibacterial peptides, anti‐inflammatory cytokines, inhibitor of κBα (IκBα) and target of rapamycin (TOR), whereas it up‐regulated the mRNA levels of pro‐inflammatory cytokines and nuclear factor kappa B p65 (NF‐κBp65) in the head kidney and spleen of fish ( p < .05). (c) Dietary PN requirements of grass carp based on the malondialdehyde content and lysozyme activity were estimated as 4.97 and 4.99 mg/kg diet, respectively.