Open AccessDietary soybean β‐conglycinin suppresses growth performance and inconsistently triggers apoptosis in the intestine of juvenile grass carp ( Ctenopharyngodon idella ) in association with ROS‐mediated MAPK signalling
Author(s) -
Duan XuDong,
Feng Lin,
Jiang WeiDan,
Wu Pei,
Liu Yang,
Kuang ShengYao,
Tang Ling,
Tang WuNeng,
Zhang YongAn,
Zhou XiaoQiu
Publication year - 2019
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12895
Subject(s) - biology , p38 mitogen activated protein kinases , grass carp , dna fragmentation , reactive oxygen species , apoptosis , mapk/erk pathway , protein kinase a , kinase , nadph oxidase , microbiology and biotechnology , medicine , biochemistry , programmed cell death , fishery , fish <actinopterygii>
The current study aimed to investigate the effects of dietary soybean β‐conglycinin on growth performance and intestine apoptosis in juvenile grass carp ( Ctenopharyngodon idella ). For fish fed with the 80 g β‐conglycinin/kg diet for 7 weeks, the specific growth rate and feed intake were decreased. In the proximal intestine, dietary β‐conglycinin did not induce DNA fragmentation, tended to decrease the reactive oxygen species (ROS) content, and decreased ROS‐generating enzyme (NADPH oxidase [NOX]) activity. Subsequently, in the mid‐intestine, dietary β‐conglycinin caused DNA fragmentation, tended to increase the ROS content, increased caspase‐3, caspase‐8 and caspase‐9 activities, upregulated the mRNA levels of proapoptotic molecules (apoptotic protease‐activating factor‐1 [ Apaf1 ] and Bcl‐2‐associated X protein [ BAX ]) and mitogen‐activated protein kinase (MAPK)‐related signal molecules (Jun N‐terminal kinase ( JNK ) and p38 MAPK ) and increased the protein levels of p38 MAPK and phospho‐p38 MAPK. Moreover, in the distal intestine, dietary β‐conglycinin induced DNA fragmentation, elevated NOX activity and the ROS content and increased caspase‐3, caspase‐8 and caspase‐9 activities, death ligand ( TNF‐α ) mRNA expression level, and p38 MAPK and phospho‐p38 MAPK protein levels. In summary, dietary soybean β‐conglycinin suppressed fish growth and inconsistently caused apoptosis among the different intestinal segments which was partially associated with ROS‐mediated MAPK signalling.