Open AccessEffects of dietary geniposidic acid on growth performance, flesh quality and collagen gene expression of grass carp, Ctenopharyngodon idella
Author(s) -
Sun W.T.,
Xu X.Y.,
Li X.Q.,
Pan W.Q.,
Leng X.J.
Publication year - 2018
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12650
Subject(s) - grass carp , flesh , biology , food science , zoology , gene expression , biochemistry , gene , fish <actinopterygii> , fishery
This study was conducted to investigate the effects of dietary geniposidic acid ( GA ) on growth performance, flesh quality and collagen gene expression of grass carp ( Ctenopharyngodon idella ). The fish with an initial body weight of 47.1 ± 0.8 g were fed one of the seven diets, including control diet, Eucommia ulmoides ( EU )‐supplemented diet (20 g/kg) and GA ‐supplemented diets (200, 400, 600, 800 and 1,000 mg/kg GA ) for 75 days. The growth performance and muscle proximate composition showed no difference among groups ( p > .05). Dietary GA (200–1,000 mg/kg) increased the contents of total collagen and alkaline‐insoluble collagen in skin ( p < .05), and high supplementation of GA (600–1,000 mg/kg GA ) and EU increased the contents of total collagen, alkaline‐insoluble collagen and total amino acids ( p < .05), but reduced the lipid level in muscle ( p < .05). In collagen gene expression, EU and 200–1,000 mg/kg GA increased COL 1A1 expression in muscle and skin ( p < .05), but the expression of COL 1A2 was increased only by high supplementation of GA (1,000 mg/kg, or 800–1,000 mg/kg) ( p < .05). In conclusion, dietary GA improved the flesh quality of grass carp, and the supplementation level was estimated to be 600 mg/kg diet.