Open AccessDietary thiamin requirement of fingerling Channa punctatus (Bloch) based on growth, protein gain, liver thiamin storage, RNA / DNA ratio and biochemical composition
Author(s) -
Zehra S.,
Khan M. A.
Publication year - 2018
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12638
Subject(s) - tbars , biology , transketolase , thiamine , thiobarbituric acid , catalase , food science , weight gain , casein , biochemistry , feed conversion ratio , zoology , enzyme , body weight , endocrinology , lipid peroxidation
Dietary thiamin requirement of fingerling Channa punctatus was quantified by feeding casein/gelatin‐based diets (450 g/kg CP ; 18.39 kJ /g GE ) with seven graded levels of thiamin (0, 0.5, 1, 1.5, 2, 2.5 and 5 mg/kg diet) to triplicate groups of fish (6.9 ± 0.93 cm; 4.91 ± 0.62 g) for 16 weeks. Fish fed diet with 2.5 mg/kg thiamin reflected highest absolute weight gain ( AWG ), protein gain ( PG ), RNA / DNA ratio and lowest feed conversion ratio. Similarly, highest liver thiamin concentration was also recorded in fish fed 2.5 mg/kg thiamin diet. Hepatic thiobarbituric acid reactive substance ( TBARS ) concentration responded negatively with increasing concentrations of dietary thiamin up to 2.5 mg/kg, whereas superoxide dismutase and catalase activities were found to improve with the increasing levels of dietary thiamin from 0 to 2.5 mg/kg. Transketolase activity also improved as the thiamin concentrations increased up to 2.5 mg/kg. Broken‐line regression analysis of AWG , PG , RNA / DNA ratio, liver thiamin concentrations, transketolase and TBARS activities exhibited the thiamin requirement in the range of 2.34–2.59 mg/kg diet. Data generated during this study would be useful in formulating thiamin‐balanced feeds for the intensive culture of this fish.