Open AccessProbiotic effect of Streptomyces spp. on shrimp ( Litopenaeus vannamei ) postlarvae challenged with Vibrio parahaemolyticus
Author(s) -
GarcíaBernal M.,
MedinaMarrero R.,
RodríguezJaramillo C.,
MarreroChang O.,
CampaCórdova Á.I.,
MedinaGarcía R.,
MazónSuástegui J.M.
Publication year - 2018
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12622
Subject(s) - litopenaeus , vibrio parahaemolyticus , biology , hepatopancreas , shrimp , artemia salina , probiotic , microbiology and biotechnology , vibrio , streptomyces , zoology , food science , toxicity , bacteria , fishery , ecology , medicine , genetics
The effect of Streptomyces strains RL8 and N7 on the growth, survival and histological structure of the hepatopancreas of Litopenaeus vannamei was determined. Shrimp post‐larvae of 0.02 ± 0.01 g were fed with diets supplemented with Streptomyces spp. RL8 and N7 at 10 8 CFU g ⁻1 of feed for 30 days, followed by challenge with Vibrio parahaemolyticus . Toxicity was also assessed by recording the mortality associated with the use of 1‐100 g/L of cell mass and 10 8 ‐10 10 CFU/g of feed of these strains in Artemia salina and L . vannamei , respectively. The group treated with Streptomyces sp. RL8 had significantly better weight gain (0.62 ± 0.18 g) and survival rates (84.44 ± 5.7%) after challenge, whereas the one fed with Streptomyces sp. N7 showed significantly higher survival rate (57.77 ± 6.8%) than the control group (0.55 ± 0.20 g and 11.11 ± 9.0%, respectively). The hepatopancreas of Streptomyces ‐fed shrimps showed better histological integrity with significantly lower degree of atrophy and necrotic tubules than their unfed counterpart. No toxicity associated with these strains was found. Consequently, they can be excellent probiotic candidates to improve the physiological status and prevent infectious diseases of cultured L. vannamei .