Open AccessGraded replacing fishmeal with canola meal in diets affects growth and target of rapamycin pathway gene expression of juvenile blunt snout bream, Megalobrama amblycephala
Author(s) -
Zhou Q.L.,
HabteTsion H.M.,
Ge X.,
Xie J.,
Ren M.,
Liu B.,
Miao L.,
Pan L.
Publication year - 2018
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12560
Subject(s) - megalobrama , biology , fish meal , feed conversion ratio , meal , zoology , gene expression , juvenile , medicine , gene , endocrinology , biochemistry , food science , fishery , fish <actinopterygii> , ecology , body weight
A feeding trial was conducted to assess the effects of replacing fishmeal (FM) with canola meal (CM) on the growth performance, feed utilization and related gene expression of juvenile blunt snout bream ( Megalobrama amblycephala ). Fishes were fed with five isonitrogenous (330 g/kg crude protein) and isoenergetic (15 MJ/kg) diets for 16 weeks, which was graded replacing levels of fishmeal (replacing 0, 37.5, 75, 112.5 and 150 g/kg FM with CM). The results revealed that specific growth rate, feed efficiency ratio and protein efficiency ratio of the groups with replacement level up to half were significantly higher than the others, while feed intake was inverse. Peptide transporter 1 (PepT1) mRNA level in the gut was significantly up‐regulated in groups CM25 and CM50 compared to other groups. Relative gene expression of IGF‐1 among groups was extremely significant based on the nonparametric test. Replacement of FM with CM caused a reduction in the hepatic expression of target of rapamycin (TOR), while significant increase trends were observed in hepatic genes of protein kinase B (AKT) and eIF4‐binding protein (4E‐BP2). However, TOR gene expression in the gut was significantly up‐regulated first and then down‐regulated, while mRNA levels of 4E‐BP2 and S6K1 in the gut were first significantly decreased and then increased in CM75, finally significantly decreased to the lowest. The overall results indicated that up to 75 g/kg FM could be replaced by CM (350.5 g/kg CM) in the diets for juvenile blunt snout bream. Besides, these signalling molecules could explain further the mechanisms that juvenile blunt snout can only maintain optimal growth performance up to 75 g/kg FM replacement with CM.