Open AccessIn vitro selection of a synbiotic and in vivo evaluation on intestinal microbiota, performance and physiological response of rainbow trout ( Oncorhynchus mykiss ) fingerlings
Author(s) -
Hoseinifar S.H.,
Mirvaghefi A.,
Amoozegar M.A.,
Merrifield D.L.,
Ringø E.
Publication year - 2017
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12373
Subject(s) - pediococcus acidilactici , fructooligosaccharide , synbiotics , biology , rainbow trout , prebiotic , food science , lactic acid , microbiology and biotechnology , probiotic , inulin , bacteria , fishery , fish <actinopterygii> , genetics , lactobacillus plantarum
The aim of this study was to determine the best synbiotic combination (based on growth and short chain fatty acids production) between Pediococcus acidilactici and five prebiotics in vitro, inulin, fructooligosaccharide ( FOS ), xylooligosaccharide ( XOS ), galactooligosaccharide ( GOS ), isomaltooligosaccharide ( IMO ). The best in vitro growth under aerobic and anaerobic culture condition was observed in the treatment, P. acidilactici + GOS . Furthermore, the study of short chain fatty acids ( SCFA ) production at the end of exponential growth phase revealed that the major SCFA produced was propionic acid in all synbiotic treatments except for the P. acidilactici + GOS treatment where butyric acid production was significantly higher compared with the other treatments. Based on the results of in vitro studies, P. acidilactici + GOS that were selected as synbiotic and rainbow trout fingerlings (15.04 ± 0.52 g) were fed on diet supplemented with the selected synbiotic. After 8 weeks of feeding, growth performance, intestinal microbiota and physiological responses were evaluated. Growth performance was significantly improved by synbiotic feeding. No significant differences of haematological parameters were revealed between the different treatments. Level of presumptive autochthonous lactic acid bacteria was elevated, while total autochthonous bacterial level remained unaffected. These results showed that P. acidilactici + GOS can be considered as an efficient synbiotic for rainbow trout.