Open AccessResponse of facilitative glucose transporter 1 to salinity stress and dietary carbohydrate nutrition in white shrimp L itopenaeus vannamei
Author(s) -
Wang X.D.,
Li E.C.,
Chen K.,
Wang S.F.,
Li T.Y.,
Xu C.,
Yu N.,
Qin J.G.,
Chen L.Q.
Publication year - 2017
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12364
Subject(s) - litopenaeus , shrimp , biology , glut1 , hepatopancreas , eyestalk , salinity , carbohydrate , glucose transporter , complementary dna , biochemistry , carbohydrate metabolism , fishery , endocrinology , ecology , gene , insulin , hormone
Abstract Facilitative glucose transporter 1 ( GLUT 1) is a transporter protein for glucose transport via the plasma membrane of the cells to provide energy through carbohydrate metabolism. GLUT 1 cDNA from Litopenaeus vannamei was obtained and analysed in this study. Full‐length GLUT 1 cDNA is 2062 bp long and contained a 1506‐bp ORF encoding a 502 amino acid protein, a 270‐bp 5′ UTR and a 284‐bp 3′ UTR . When shrimp were under acute low salinity stress, the expression in hepatopancreas, muscle, gill and eyestalk was all up‐regulated at 12 h ( P < 0.05) and 96 h ( P < 0.05), while the expression in the four tissues was all down‐regulated at 6 h ( P < 0.05) and 48 h ( P < 0.05) . The expression in the muscle of shrimp at water salinity of 3 was lower than that at water salinity of 30 independent of dietary carbohydrate levels, while expression in hepatopancreas, gill and eyestalk was up‐regulated at 200 and 300 g kg −1 carbohydrate levels. The expression in all tissues fed glucose was up‐regulated when compared to the expression in shrimp held at a water salinity of 30. This study suggests that GLUT 1 is a conserved protein in L. vannamei , and changes in expression due to environmental salinity and dietary carbohydrate level and source.