Open AccessDietary leucine requirement of Juvenile Nile tilapia, Oreochromis niloticus
Author(s) -
Gan L.,
Zhou L.L.,
Li X.X.,
Yue Y.R.
Publication year - 2016
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12353
Subject(s) - nile tilapia , leucine , oreochromis , biology , juvenile , weight gain , lysozyme , zoology , tilapia , composition (language) , feed conversion ratio , food science , medicine , body weight , biochemistry , endocrinology , amino acid , fish <actinopterygii> , ecology , fishery , linguistics , philosophy
An 8‐week feeding trial was conducted to evaluate the effects of dietary leucine on growth performance, feed utilization, body composition and non‐specific immune responses of juvenile Nile tilapia. Five isonitrogenous and isoenergetic diets were formulated to contain graded levels of L‐leucine (5.3, 8.1, 10.9, 13.2, 15.6 and 18.1 g kg −1 diet, respectively) from dietary ingredients and crystalline L‐leucine. Each diet was randomly assigned to triplicate groups of 20 juvenile fish (1.94 ± 0.01 g) three times daily to apparent satiation. Results showed that the weight gain ( WG ) and specific growth rate ( SGR ) increased as dietary leucine concentrations increased from 5.3 to 13.2 g kg −1 and then decreased slightly with further increase in dietary leucine concentrations. Quadratic regression analysis ( y = −522.6 x 2 + 1304. x + 132.6, R ² = 0.684) on weight gain against dietary leucine levels indicated that the optimal dietary leucine requirement was estimated to be 12.5 g kg −1 diet (corresponding to 43.1 g kg −1 of dietary protein). Leucine supplementation had no impact on the survival and body composition of tilapia. Serum lysozyme activity of fish fed diet containing 13.2 g kg −1 leucine significantly increased compared to fish fed diet containing 5.3 g kg −1 . Serum superoxide dismutase activity and immunoglobulin M (IgM) concentration were not significantly affected by dietary leucine supplementation.