Open AccessOptimal dietary alpha‐linolenic acid/linoleic acid ratio improved digestive and absorptive capacities and target of rapamycin gene expression of juvenile grass carp ( C tenopharyngodon idellus )
Author(s) -
Zeng Y.Y.,
Jiang W.D.,
Liu Y.,
Wu P.,
Zhao J.,
Jiang J.,
Kuang S.Y.,
Tang L.,
Tang W.N.,
Zhang Y.A.,
Zhou X.Q.,
Feng L.
Publication year - 2016
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12337
Subject(s) - biology , grass carp , hepatopancreas , medicine , endocrinology , feed conversion ratio , macrobrachium rosenbergii , amylase , biochemistry , enzyme , fishery , fish <actinopterygii> , body weight , prawn
Growth performance, digestive and absorptive capacities and target of rapamycin ( TOR ), ribosomal protein S6 kinase 1 (S6K1) and eIF 4E‐binding protein (4E‐ BP ) gene expression in the hepatopancreas and intestine of juvenile grass carp ( C tenopharyngodon idellus ) fed graded ratios of dietary alpha‐linolenic acid/linoleic acid ( ALA / LNA ) (0.01, 0.34, 0.68, 1.03, 1.41, 1.76 and 2.15) for 60 days were investigated. The results showed that ALA / LNA ratio of 1.03 significantly improved (i) per cent weight gain ( PWG ) and feed efficiency, (ii) hepatopancreatic trypsin, chymotrypsin, lipase, amylase and intestinal creatine kinase ( CK ) activities, (iii) hepatopancreatic trypsinogen‐2 and chymotrypsinogen mRNA levels. Meanwhile, fish fed with ALA / LNA ratio of 0.68 significantly enhanced, (iv) Na + /K + ‐ ATP ase and γ‐glutamyl transpeptidase activities in whole intestine, and alkaline phosphatase activities in the proximal intestine (PI) and distal intestine, (v) amylase, intestinal Na + /K + ‐ ATP ase alpha‐subunit isoform 1, Na + /K + ‐ ATP ase alpha‐subunit isoform 8 and CK mRNA abundances, (vi) TOR and S6K1 gene expression in the hepatopancreas and intestine of juvenile grass carp. Based on the quadratic regression analysis of PWG , cholecystokinin and leptin contents in the PI, optimal dietary ALA / LNA ratio of juvenile grass carp (8.78–72.00 g) was estimated to be 1.08, 1.19 and 1.05, respectively.