Open AccessEffects of dietary glucose and starch levels on the growth, haematological indices and hepatic hexokinase and glucokinase m RNA expression of juvenile mirror carp ( C yprinus carpio )
Author(s) -
Li J.N.,
Xu Q.Y.,
Wang C.A.,
Wang L.S.,
Zhao Z.G.,
Luo L.
Publication year - 2016
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12278
Subject(s) - glucokinase , biology , protein efficiency ratio , postprandial , starch , cyprinus , glycogen , carbohydrate , carp , hexokinase , feed conversion ratio , medicine , carbohydrate metabolism , endocrinology , food science , zoology , biochemistry , metabolism , insulin , fish <actinopterygii> , glycolysis , fishery , body weight
The effect of the different dietary carbohydrate types and levels on growth performance, haematological indices and hepatic hexokinase ( HK ) and glucokinase ( GK ) genes expression involved in control of glucose metabolism, was studied in juvenile mirror carp ( C yprinus carpio ). Two carbohydrates (glucose and starch) diets with two levels (250 and 500 g kg −1 ) were fed to triplicate groups of 35 fish for 60 days. The best weight gain rate and specific growth rate were observed in fish fed with 250 g kg −1 glucose diet and 500 g kg −1 starch diet ( P < 0.05). Fish fed with 500 g kg −1 glucose showed low feed utilization, with the highest food conversion ratio and the lowest protein efficiency ratio ( P < 0.05). Hepatosomatic index was significantly higher in fish fed with glucose diets and the 500 g kg −1 starch diet compared to 250 g kg −1 starch. CHOL , HDL ‐ C and LDL ‐ C were significantly highest in fish fed with 500 g kg −1 starch than all other diets ( P < 0.05). Hepatic GK m RNA expression level and activity were positively related to glucose and starch levels ( P < 0.05). Correlation analysis showed that hepatic glycogen concentration was increased by dietary carbohydrate content ( P < 0.05). These results suggest that GK may play a major role in the postprandial glucose utilization in juvenile mirror carp.