Open AccessEffects of dietary phenylalanine on growth, digestive and brush border enzyme activities and antioxidant capacity in the hepatopancreas and intestine of young grass carp ( Ctenopharyngodon idella )
Author(s) -
Li W.,
Feng L.,
Liu Y.,
Jiang W.D.,
Kuang S.Y.,
Jiang J.,
Li S.H.,
Tang L.,
Zhou X.Q.
Publication year - 2015
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12223
Subject(s) - hepatopancreas , grass carp , biology , amylase , digestive enzyme , phenylalanine , antioxidant , protease , superoxide dismutase , glutathione peroxidase , medicine , food science , endocrinology , biochemistry , enzyme , fishery , amino acid , fish <actinopterygii>
This study investigated the effects of phenylalanine on growth, digestive and absorptive ability and antioxidant status of young grass carp ( Ctenopharyngodon idella ). Young grass carp were fed diets containing 3.4 (basal diet), 6.1, 9.1, 11.5, 14.0 and 16.8 g phenylalanine kg −1 diet with a fixed of 10.7 g tyrosine kg −1 diet for 8 weeks. Percent weight gain ( PWG ), feed efficiency and feed intake of fish were the lowest in fish fed the basal diet ( P < 0.05). Trypsin, lipase and amylase activities in the hepatopancreas, and antioxidants including glutathione contents and glutathione reducase activities in the hepatopancreas and intestine were all the highest in fish fed 11.5 g phenylalanine kg −1 diet ( P < 0.05). Trypsin, chymotrypsin and amylase activities in whole intestine, and creatine kinase, Na + , K + ‐ ATP ase and alkaline phosphatase activities in the proximal intestine, and superoxide dismutase activities in the hepatopancreas and intestine were all the highest when phenylalanine at level of 9.1 g kg −1 diet ( P < 0.05). In conclusion, phenylalanine improved growth, digestive and absorptive ability, and antioxidant capacity of young grass carp. The phenylalanine requirement of young grass carp (256–629 g) based on PWG was 10.4 g kg −1 diet or 3.44 g 100 g −1 protein.