Open AccessImmune responses and gut morphology of S enegalese sole ( S olea senegalensis , K aup 1858) fed monospecies and multispecies probiotics
Author(s) -
Batista S.,
Ramos M.A.,
Cunha S.,
Barros R.,
Cristóvão B.,
Rema P.,
Pires M.A.,
Valente L.M.P.,
Ozório R.O.A.
Publication year - 2015
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12191
Subject(s) - pediococcus acidilactici , biology , probiotic , food science , prebiotic , immune system , lactobacillus , pediococcus , zoology , microbiology and biotechnology , bacteria , lactobacillus plantarum , lactic acid , immunology , genetics , fermentation
The current study aimed to determine the effects of dietary probiotic supplementation on growth, gut morphology and non‐specific immune parameters in Senegalese sole ( Solea senegalensis ) juveniles during a 1‐month trial. Fish were fed for 1‐month two diets with 1.0 or 4.6 × 10 6 CFU kg −1 ) of probiotic A ( Bacillus sp., Pediococcus sp., Enterococcus sp. and Lactobacillus sp.) and two diets with 3.5 or 8.6 × 10 5 CFU kg −1 of probiotic B ( Pediococcus acidilactici ) and tested against an unsupplemented diet (control). Growth performance, as well as respiratory burst activity, nitric oxide ( NO ), alternative complement pathway ( ACH 50), lysozyme and peroxidase activities, was not affected by the dietary treatments. Probiotic supplementation tended to increased growth homogeneity between tanks having diet A 1 the best possible alternative to decrease costs associated to size grading. Villous length and number of goblet cells of the anterior intestine did not vary among treatments. Muscle duodenal layer was significantly thicker in fish fed probiotic A compared to probiotic B, when included at the lowest level (A 2 versus B 2 ). The current study indicate that the use of the multispecies probiotic at 1.0 × 10 6 CFU kg −1 might enhance protection against pathogen outbreak and increase nutrient absorption, whereas at the highest concentration could reduced size dispersion among tanks.