Open AccessEffects of fish oil replacement by a vegetable oil blend on digestibility, postprandial serum metabolite profile, lipid and glucose metabolism of E uropean sea bass ( D icentrarchus labrax ) juveniles
Author(s) -
Castro C.,
Corraze G.,
Panserat S.,
OlivaTeles A.
Publication year - 2015
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12184
Subject(s) - dicentrarchus , fish oil , postprandial , lipid metabolism , biology , polyunsaturated fatty acid , microsomal triglyceride transfer protein , fish meal , biochemistry , food science , medicine , endocrinology , lipoprotein , cholesterol , very low density lipoprotein , insulin , fatty acid , fishery , fish <actinopterygii>
A digestibility trial was conducted to evaluate the effects of dietary fish oil ( FO ) replacement by a vegetable oil blend ( VO ) (rapeseed, linseed and palm oils) on nutrient digestibility, lipid absorption and transport and lipid and carbohydrate metabolic pathways of D icentrarchus labrax juveniles. Following 36 days, the liver and intestine of fish were sampled 6 h after the last meal. Remaining fish were fed one more week and blood was collected at different postprandial time intervals. Dietary VO decreased apparent digestibility coefficients (ADC) of lipid and energy. Among the serum parameters analysed, only high‐density lipoprotein was affected, being higher in VO group. Hepatic and intestinal expression of genes related to lipid transport and lipoprotein metabolism ( FABP , DGAT , ApoB, ApoA4, ApoA1, MTP ) showed no differences in transcript levels. No alteration in genes related to cholesterol biosynthesis ( HMGCR ), glucose transport ( GLUT 2) or metabolism ( GK , PK , G6Pase and PEPCK ) in the liver and intestine was found. However, hepatic transcript levels of FADS2 and elovl5 were up‐regulated in VO group. In conclusion, replacement of FO by VO did not induce major alterations at molecular and biochemical levels, although a nutritional modulation of LC ‐polyunsaturated fatty acids biosynthesis pathway was observed.