Open AccessTarget of rapamycin ( TOR ) in F enneropenaeus chinensis : cDNA cloning, characterization, tissue expression and response to amino acids
Author(s) -
Sun S.J.,
Wang B.J.,
Jiang K.Y.,
Sun J.,
Liu M.,
Wang L.
Publication year - 2015
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12133
Subject(s) - biology , complementary dna , cloning (programming) , molecular cloning , microbiology and biotechnology , biochemistry , gene , computer science , programming language
Target of rapamycin ( TOR ) kinase is a central cell growth controller that integrates a wide array of extracellular and intracellular signals to regulate cell growth. In this study, the TOR gene of F enneropenaeus chinensis , an important aquatic species in C hina, was cloned and characterized. The cDNA of fch‐ TOR comprises 7638 bp and contains a 7389‐bp open reading frame, which encodes a peptide of 2462 amino acids. The deduced amino acid sequence of fch‐ TOR contains the characteristic rapamycin‐binding domain and PI3Kc domain. The fch‐ TOR gene was mainly expressed in muscles. When leucine or arginine was supplied to hungry shrimps, the expression of fch‐ TOR was significantly enhanced and the phosphorylation of protein factors including fch‐ TOR and S6K1 in the TOR signalling pathway was significantly promoted. The results indicated that leucine and arginine could stimulate the expression of fch‐ TOR and activate the TOR signalling pathway and that the gene transcription response lagged behind protein phosphorylation.