Open AccessDietary biotin requirement of juvenile blunt snout bream, M egalobrama amblycephala
Author(s) -
Qian Y.,
Li X.F.,
Sun C.X.,
Jiang G.Z.,
Xu W.N.,
Wang Y.,
Liu W.B.
Publication year - 2014
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12114
Subject(s) - megalobrama , biotin , biology , pyruvate carboxylase , feed conversion ratio , protein efficiency ratio , zoology , endocrinology , medicine , body weight , biochemistry , fish <actinopterygii> , enzyme , fishery
This study was conducted to determine the optimal dietary biotin requirement of juvenile M egalobrama amblycephala . Quadruple groups of fish (initial average weight 2.01 ± 0.01 g) were fed thrice daily with six isonitrogenous and isoenergetic purified diets containing 0 (basal diet), 0.015, 0.049, 0.158, 0.624 and 2.49 mg kg −1 biotin, respectively, for 8 weeks. Results showed that survival rate, final weight, feed intake, specific growth rate, protein efficiency ratio and nitrogen retention efficiency all increased significantly ( P < 0.01) as dietary biotin levels increased from 0 to 0.049 mg kg −1 , whereas the opposite was true for feed conversion ratio. Dressout percentage, condition factor, hepatosomatic index, viscera/body ratio all showed no significant difference ( P > 0.05) within the biotin range tested. Contrary to moisture content, whole‐body protein and lipid contents showed a positive correlation with dietary biotin levels. In addition, liver biotin content increased significantly ( P < 0.05) with increasing dietary biotin levels up to 0.624 mg kg −1 . Hepatic pyruvate carboxylase ( PC ) and acetyl‐ C o A carboxylase ( ACC ) activities both showed an increasing trend as dietary biotin levels increased. Based on the regression analysis of weight gain, hepatic PC and ACC activities, the optimal dietary biotin requirement of juvenile M egalobrama amblycephala is estimated to be 0.063, 0.071 and 0.075 mg kg −1 , respectively.