z-logo
open-access-imgOpen Access
Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp ( C arassius auratus gibelio )
Author(s) -
Chen Y.,
Zhu X.,
Yang Y.,
Han D.,
Jin J.,
Xie S.
Publication year - 2014
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12106
Subject(s) - carp , biology , chitosan , respiratory burst , microbiology and biotechnology , aeromonas , aeromonas hydrophila , aeromonas veronii , immune system , immunostimulant , zoology , medicine , bacteria , immunology , biochemistry , fish <actinopterygii> , fishery , genetics
A 75‐day experiment was conducted with juvenile gibel carp ( C arassius auratus gibelio ) (4.80 ± 0.01 g) to evaluate effects of dietary chitosan on fish growth performance, haematology, intestine morphology and immune response. Six isonitrogenous (crude protein: 383 g kg −1 ), isolipid (97.5 g kg −1 ) and isocaloric (gross energy: 16.7 kJ g −1 ) diets were formulated to contain 0, 1800, 4000, 7500, 10 000, 20 000 mg kg −1 chitosan, respectively. The results showed that the growth was depressed when the fish fed with 10 000 mg kg −1 chitosan. Serum cholesterol, triglyceride and low‐density lipoprotein decreased in 10 000 and 20 000 mg kg −1 chitosan. On day 75, blood leucocyte phagocytic activity respiratory burst and alternative pathway of complement haemolytic activity were enhanced in 4000 mg kg −1 chitosan. The number of goblet cell, intraepithelial lymphocyte of mid‐intestine and microvilli height of distal intestine increased at 4000 mg kg −1 dietary chitosan. Dietary chitosan modulated intestine microbiota, depressed pathogen bacteria A eromonas veronii‐ like and improved C ellulomonas hominis ‐like, B acillus oceanisediminis ‐like and two uncultured bacterium‐like species on day 75. Dietary 7500 and 10 000 mg kg −1 chitosan enhanced the protection against A eromonas hydrophila infection. In conclusion, oral administration of dietary 7500 mg kg −1 chitosan for 75 days is recommended for the survival of gibel carp.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here