Open AccessPhysical treatment of high EPA Y arrowia lipolytica biomass increases the availability of n‐3 highly unsaturated fatty acids when fed to A tlantic salmon
Author(s) -
Berge G.M.,
Hatlen B.,
Odom J.M.,
Ruyter B.
Publication year - 2013
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12092
Subject(s) - yarrowia , biology , food science , eicosapentaenoic acid , yeast , biomass (ecology) , docosahexaenoic acid , bioavailability , fish meal , fatty acid , ingredient , polyunsaturated fatty acid , salmo , biochemistry , fish <actinopterygii> , fishery , agronomy , bioinformatics
A strain of Y arrowia lipolytica engineered to produce high levels of eicosapentaenoic acid ( EPA ) was used as feed ingredient to A tlantic salmon ( S almo salar L .). The study was designed to investigate the effect of washing and cell disruption, on growth, bioavailability, and fillet composition of A tlantic salmon growing from 0.5 to 1 kg. Four diets containing 200 g kg ‐1 yeast ( U nwashed/ N ot disrupted, U nwashed/ D isrupted, W ashed/ U ndisrupted, W ashed/ D isrupted) were compared to a fishmeal‐based control diet in a 112‐day feeding experiment. Final weights and growth rates were similar for all dietary groups. Apparent lipid digestibility was highly affected by disruption of the yeast biomass. Disruption increased the apparent digestibility of EPA from 32% to 76% in washed biomass, and from 26% to 76% in U nwashed biomass. Sum n‐3 fatty acids deposited in whole body was similar in fish fed the control diet and the diets with D isrupted yeast biomass, while fish fed U ndisrupted biomass had significantly lower content of n‐3. Removal of residual medium components by washing had no discernible effect on utilization of EPA . Evidence for conversion of EPA to DHA (docosahexaenoic acid) in the fish was indicated by deposition ratio R d < 1 for EPA and R d > 1 for DHA .