Open AccessEffects of dietary astaxanthin on growth, antioxidant capacity and gene expression in P acific white shrimp L itopenaeus vannamei
Author(s) -
Zhang J.,
Liu Y.J.,
Tian L.X.,
Yang H.J.,
Liang G.Y.,
Yue Y.R.,
Xu D.H.
Publication year - 2013
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/anu.12037
Subject(s) - shrimp , litopenaeus , biology , astaxanthin , superoxide dismutase , penaeidae , hypoxia (environmental) , zoology , catalase , antioxidant , food science , fishery , decapoda , biochemistry , crustacean , oxygen , chemistry , carotenoid , organic chemistry
Pacific white shrimp L itopenaeus vannamei (1050 individuals with initial weight of 1.01 ± 0.001 g) were fed either control diet or one of six dietary astaxanthin ( AX ) concentration (25, 50, 75, 100, 125 and 150 mg kg −1 ) diets for 56 days in 35 tanks (30 shrimp per tank). After 56 days of culture, shrimp‐fed AX 125 and AX 150 diets had higher ( P < 0.05) weight gain, specific growth rate, total antioxidant status and lower ( P < 0.05) superoxide dismutase ( SOD ), catalase ( CAT ) than shrimp fed control diet. After low dissolved oxygen stress for 1 h, survival rate of shrimp fed AX 75, AX 100, AX 125 and AX 150 diets was higher ( P < 0.05) than that of shrimp fed control diet. Hypoxia inducible factor‐1α ( HIF ‐1α), cytosolic manganese superoxide dismutase ( cMnSOD ) and CAT mRNA expression levels of shrimp fed seven diets were significantly down‐regulated under hypoxia than under normoxia, but their expression levels were higher under hypoxia in shrimp fed AX‐ supplemented diets than in shrimp fed control diet. About 70‐kDa heat‐shock protein ( H sp70) mRNA expression level of shrimp fed seven diets was significantly up‐regulated under hypoxia than under normoxia, but its expression level was lower under hypoxia in shrimp fed AX ‐supplemented diets than in shrimp fed control diet.