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Low prevalence of human papillomavirus in oral cavity squamous cell carcinoma in Queensland, Australia
Author(s) -
Emmett Sarah,
Jenkins Glenn,
Boros Samuel,
Whiteman David C.,
Panizza Benedict,
Antonsson Annika
Publication year - 2017
Publication title -
anz journal of surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 70
eISSN - 1445-2197
pISSN - 1445-1433
DOI - 10.1111/ans.13607
Subject(s) - medicine , polymerase chain reaction , human papillomavirus , immunohistochemistry , cyclin d1 , etiology , pathology , oral cavity , hpv infection , population , carcinoma , basal cell , oncology , cancer , cell cycle , cervical cancer , gene , biology , dentistry , biochemistry , environmental health
Background While human papillomavirus ( HPV ) is an accepted risk factor for oropharyngeal squamous cell carcinoma ( SCC ), its aetiological role in oral cavity SCC remains unclear. This study aimed to determine the HPV prevalence in an Australian population. Methods DNA was extracted from 63 formalin‐fixed paraffin‐embedded tumour specimens histologically confirmed as SCC of the oral cavity, diagnosed during 2006–2012. Clinical data were extracted from medical records. HPV presence was determined by polymerase chain reaction. Positive samples were typed by sequencing. Immunohistochemistry was used to assess p16 INK4A , p53, pRB , Ki67, Cyclin D1 and p21 WAF1 expression. Results Five of the 63 tumours (8%) were positive for HPV DNA (three HPV ‐16 positive and two HPV ‐18 positive). Two tumours overexpressed p16 INK4A (3%) and one of these was also HPV positive. Overexpression of Cyclin D1 correlated significantly with tumour recurrence ( P = 0.029) and death ( P = 0.002). Conclusions This study has identified a low prevalence of high‐risk HPV in Queensland, Australia.