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In vitro effect of autologous platelet‐rich plasma on H 2 O 2 ‐induced oxidative stress in human spermatozoa
Author(s) -
Bader R.,
Ibrahim J. N.,
Moussa M.,
Mourad A.,
Azoury J.,
Azoury J.,
Alaaeddine N.
Publication year - 2020
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12648
Subject(s) - vacuolization , andrology , dna fragmentation , sperm , oxidative stress , reactive oxygen species , platelet rich plasma , motility , sperm motility , semen , fragmentation (computing) , chemistry , semen analysis , male infertility , biology , platelet , apoptosis , immunology , endocrinology , medicine , infertility , microbiology and biotechnology , biochemistry , programmed cell death , genetics , ecology , pregnancy
Background Among new therapies emerging in the medical field, the use of platelet‐rich plasma ( PRP ) in human reproduction has not yet been explored. Objectives This study aimed at investigating the effect of autologous PRP on sperm parameters in the presence and absence of H 2 O 2 . Materials and methods Semen samples were collected from 30 healthy men in their fourth decade attending Azoury IVF clinic. Spermatozoa, cultured in the presence or absence of 10 μM H 2 O 2 , were left untreated or treated with increasing concentrations of PRP (2, 5, and 10%). After 24‐h incubation, ROS levels were assessed and sperm parameters were evaluated. Results Our results highlight the harmful effect of H 2 O 2 on sperm parameters, showing an increase in the percentage of reactive oxygen species ( ROS )‐positive cells, vacuolization, and sperm DNA fragmentation, and a decrease in progressive and total motility in the H 2 O 2 ‐treated group compared to non‐stressed spermatozoa. When samples were treated with PRP , an improvement of the studied parameters was noted mainly with 2% PRP , thus regarded as the best concentration to achieve a positive effect on sperm parameters. Indeed, non‐stressed and stressed spermatozoa treated with 2% PRP showed a significant increase in progressive and total motility, coupled with a decrease in ROS ‐positive cells, DNA fragmentation, vacuolization, and dead cells compared to the untreated group. In contrast, no significant difference in cell morphology was found between the two groups. Moreover, 2% PRP treatment enhanced sperm parameters and prevented cell death in H 2 O 2 ‐exposed spermatozoa as compared to freshly collected semen. Discussion We suggest that PRP because of its wide arrays of growth factors included in his alpha granules contributes to the inhibition of ROS through the antioxidant, anti‐apoptotic activity. Conclusion Autologous PRP improves the quality of the sperm, more so in the presence of an H 2 O 2 ‐induced OS .