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Immunolocalization of DMRTB1 in human testis with normal and impaired spermatogenesis
Author(s) -
Hilbold E.,
Bergmann M.,
Fietz D.,
Kliesch S.,
Weidner W.,
Langeheine M.,
Rode K.,
Brehm R.
Publication year - 2019
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12617
Subject(s) - spermatogenesis , sertoli cell , immunostaining , biology , immunohistochemistry , seminoma , germ cell , andrology , mitosis , staining , testicle , pathology , endocrinology , microbiology and biotechnology , immunology , medicine , genetics , gene , chemotherapy
Background The transcription factor DMRTB 1 plays a pivotal role in coordinating the transition between mitosis and meiosis in murine germ cells. No reliable data are available for human testis. Objectives The present study aims to examine the testicular expression pattern of DMRTB 1 in men showing normal and impaired spermatogenesis. Materials and methods Immunohistochemistry was performed using 54 human testicular biopsy specimens and a commercial rabbit polyclonal anti‐ DMRTB 1 primary antibody. RT ‐ PCR complemented immunohistochemistry. To further characterize immunopositive cells and possible co‐localization, the proliferation marker Ki‐67, the tumor marker PLAP , and an anti‐ DMRT 1 antibody were used. Results In men with normal spermatogenesis, a strong immunoreactivity was detectable in a subset of spermatogonia (38.34 ± 2.14%). Some spermatocytes showed a weak immunostaining. Adjacent Sertoli cells were immunonegative. Compared with a hematoxylin and eosin overview staining, these immunopositive cells were almost exclusively identified as A pale and B spermatogonia and primary spermatocytes in (pre‐)leptotene, zygotene, and pachytene stages. In patients with spermatogenic arrest at spermatogonial level, an altered staining pattern was found. No immunoreactivity was detected in Sertoli cells in Sertoli cell‐only syndrome. In germ cell neoplasia in situ ( GCNIS ) tubules, except for a few (0.4 ± 0.03%), pre‐invasive tumor cells were immunonegative. Seminoma cells showed no immunostaining. Discussion According to previous findings in mice, it seems reasonable that DMRTB 1 is expressed in these normal germ cell populations. Moreover, altered staining pattern in spermatogenic arrest at spermatogonial stage suggests a correlation with mitosis and transformation into B spermatogonia. The absence of DMRTB 1 in GCNIS cells and tumor cells might be associated with uncontrolled neoplastic cell proliferation and progression into invasive germ cell tumors. Further research is required to elucidate, for example, the role of DMRTB 1 in the malignant transformation of human germ cells. Conclusion Our data indicate a relevant role for DMRTB 1 regarding the entry of spermatogonia into meiosis in men.