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Among seven testis‐specific molecular markers, SPEM 1 appears to have a significant clinical value for prediction of sperm retrieval in azoospermic men
Author(s) -
Hashemi M.S.,
Mozdarani H.,
Ghaedi K.,
NasrEsfahani M. H.
Publication year - 2018
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12528
Subject(s) - sperm retrieval , testicular sperm extraction , azoospermia , andrology , intracytoplasmic sperm injection , receiver operating characteristic , meiosis , biology , spermatogenesis , sperm , obstructive azoospermia , male infertility , gene , medicine , genetics , embryo , infertility , in vitro fertilisation , pregnancy
Background To achieve sperm retrieval in azoospermic men, predicting the success rate seems to be necessary. Objectives In the present study we aimed to assess expression of seven molecular markers [ ESX 1, DAZ , DAZL (pre‐meiotic markers), ZMYND 15, PRM 1, TNP 1, and SPEM 1 (post‐meiotic markers)] to predict the success of sperm retrieval. Materials and Methods In this study, 63 azoospermic men [16 OA (obstructive azoospermia) and 47 NOA (nonobstructive azoospermia)] undergoing testicular tissue microdissection (micro‐ TESE ) for intracytoplasmic sperm injection ( ICSI ). Expression levels of these target genes were determined by real‐time reverse transcription polymerase chain reaction using the DDC t method, and efficacy of each gene was compared by receiver operating characteristic ( ROC ) curve analysis. Results Expression of post‐meiotic transcripts significantly decreases in NOA and its subgroups ( SCOS : Sertoli cell only syndrome, MA : maturation arrest, and HS : hypospermatogenesis) with spermatogenic failure compared to normal spermatogenesis ( OA ), with an exception of ZMYND 15 for the HS group. These findings suggest the differential expression of the post‐meiotic ZMYND 15 marker is in accordance with histological findings and can discriminate HS from SCOS and MA . Post‐meiotic markers were significantly reduced in negative vs. positive sperm retrieval groups. Discussion and Conclusion Among the seven markers, SPEM 1 had the best positive prediction power (96%) and negative prediction power (85%) at a 0.086 cutoff with the area under the curve ( AUC ) of 0.91 for receiver operating characteristic 4 ( ROC ) to predict the micro‐ TESE outcome.