Premium
sh RNA mediated ablation of prostate and testis expressed ( Pate ) messenger RNA results in impaired sperm function and fertility
Author(s) -
Rajesh A.,
Yenugu S.
Publication year - 2017
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12321
Subject(s) - capacitation , transfection , sperm , rna , messenger rna , biology , microbiology and biotechnology , spermatogenesis , gene , epididymis , gene expression , andrology , genetics , endocrinology , medicine
Summary Spermatogenesis and sperm maturation are complex processes mediated by a variety of proteins present in the testicular and epididymal mileu. In the recent years, functional characterization of these proteins is being studied by genetic manipulations that involve targeted over expression or knock down of specific genes. In this study, we adopted FuGENE 6‐based in vivo transfection of rat cauda epididymis with p GFP ‐V‐ RS plasmid that encodes sh RNA to knock down Pate m RNA levels to implicate a possible role for this gene in sperm function. The m RNA levels of Pate gene were significantly decreased in HEK cells as well as in cauda of rats upon transfection with p GFP ‐V‐ RS plasmid that encodes sh RNA targeting Pate gene. Spermatozoa obtained from the transfected cauda displayed impairment in capacitation and acrosome reaction. Furthermore, rats subjected to in vivo transfection to knock down Pate m RNA levels had compromised fertility. Results of our study indicate a role for Pate gene in sperm function and can be exploited as a potential male contraceptive target.