Premium
Papaverine‐sensitive phosphodiesterase activity is measured in bovine spermatozoa
Author(s) -
Bergeron A.,
Hébert A.,
Guillemette C.,
Laroche A.,
Poulin M.P.,
Aragon J. P.,
Leclerc P.,
Sullivan R.,
Blondin P.,
Vigneault C.,
Richard F. J.
Publication year - 2017
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12290
Subject(s) - capacitation , papaverine , phosphodiesterase , acrosome reaction , motility , sperm motility , sperm , chemistry , andrology , pde10a , medicine , endocrinology , biology , microbiology and biotechnology , enzyme , biochemistry
Summary Cyclic adenosine monophosphate ( cAMP ) plays a crucial role as a signaling molecule for capacitation, motility, and acrosome reaction in mammalian spermatozoa. It is well‐known that cAMP degradation by phosphodiesterase ( PDE ) enzyme has a major impact on sperm functions. This study was undertaken to characterize cAMP ‐ PDE activity in bovine spermatozoa. Total cAMP ‐ PDE activity in cauda epididymal and ejaculated spermatozoa was 543.2 ± 49.5 and 1252.6 ± 86.5 fmoles/min/10 6 spermatozoa, respectively. Using different family‐specific PDE inhibitors, we showed that in cauda epididymal and ejaculated spermatozoa, the major cAMP ‐ PDE activity was papaverine‐sensitive (44.5% and 57.5%, respectively, at 400 n m , papaverine is a specific inhibitor of the PDE 10 family). These data are supporting the functional presence of PDE 10 in bovine spermatozoa and were further confirmed by western blot to be PDE 10A. Using immunocytochemistry, we showed immunoreactive signal for PDE 10A present on the post‐acrosomal region of the head and on the flagella of ejaculated spermatozoa. Using papaverine, we showed that it promotes tyrosine phosphorylation of sperm proteins, phosphorylation of Erk1 and Erk2, and Ca 2+ release from Ca 2+ store. These results suggest that PDE 10 is functionally present in bovine spermatozoa and is affecting different molecular events involved in capacitation, most probably by cAMP local regulation.