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Expression ratio of histone demethylase KDM 3A to protamine‐1 mRNA is predictive of successful testicular sperm extraction in men with obstructive and non‐obstructive azoospermia
Author(s) -
Javadirad S. M.,
Hojati Z.,
Ghaedi K.,
NasrEsfahani M. H.
Publication year - 2016
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12164
Subject(s) - obstructive azoospermia , testicular sperm extraction , sperm retrieval , azoospermia , andrology , sperm , protamine , biology , medicine , infertility , genetics , pregnancy , heparin
Summary To evaluate the predictive value of histone demethylase KDM3A to protamine 1 (PRM1) mRNA expression ratio as a reliable marker of sperm retrieval in men with obstructive and non‐obstructive azoospermia (NOA). Fifty eight azoospermic men, including 44 with NOA and 14 with obstructive azoospermia ( OA ). Testis tissue samples were collected from azoospermic men who have been referred for testicular sperm extraction (TESE) and micro‐ TESE . Relative expression ratio of KDM3A to PRM1 was analyzed after selection of approved reference genes. Histological classification of testis biopsies was performed. Sperm retrieval following TESE and micro‐ TESE was evaluated. A sperm retrieval prediction sensitivity of 95% was established when the Cq of PRM1 became smaller than the Cq of both KDM3A and GAPDH genes. However, azoospermic men with down‐regulated KDM 3A and decreased expression of PRM 1 mRNA showed very low success for sperm retrieval (<25%), even after micro‐ TESE surgery. The KDM3A to PRM1 mRNA expression ratio can be used as a reliable marker of successful testicular sperm extraction in men with obstructive and non‐obstructive azoospermia with 95% sensitivity.