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Restoration of erectile function with intracavernous injections of endothelial progenitor cells after bilateral cavernous nerve injury in rats
Author(s) -
Liao C.H.,
Wu Y.N.,
Lin Y.H.,
Syu Huang R.F.,
Liu S.P.,
Chiang H.S.
Publication year - 2015
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12085
Subject(s) - enos , medicine , erectile dysfunction , progenitor cell , von willebrand factor , nerve injury , intracavernous injection , penis , endothelium , urology , mean arterial pressure , endothelial progenitor cell , endocrinology , nitric oxide , anesthesia , nitric oxide synthase , surgery , blood pressure , stem cell , platelet , biology , heart rate , genetics
Summary Endothelial progenitor cells ( EPC s) are bone marrow‐derived endothelial cells capable of circulating, proliferating, and differentiating into mature endothelial cells. Circulating EPC s can be directly recruited to some extent at sites of injury, and their administration could accelerate repair or endothelialization of the damaged tissue. We investigated the effects of intracavernous injections of EPC s into the corpora cavernosa of rats with erectile dysfunction ( ED ) caused by bilateral cavernous nerve ( CN ) injury. Overall, 24 male Sprague–Dawley rats were randomized into three groups: sham surgery, vehicle‐only, or EPC treatment. Rats in the EPC treatment and vehicle‐only groups were subjected to bilateral CN injury before injection of EPC s or vehicle, respectively, into the corpora cavernosa. Four weeks after surgery, erectile function was assessed by measuring maximum intracavernosal pressure ( ICP ), change in ICP , area under the ICP curve, and ratio of change in ICP and mean arterial pressure ( MAP ; Δ ICP / MAP ). Penile tissue was histomorphometrically analyzed for the expression of neural nitric oxide synthase ( nNOS ), neurofilament‐1 ( NF ‐1), von Willebrand factor ( vWF ), endothelial NOS ( eNOS ), and smooth muscle cell content. Maximum ICP and all other functional parameters of erectile function were significantly reduced in the vehicle‐only group vs. the sham and EPC treatment groups (all p < 0.001). Smooth muscle cell content was decreased in the vehicle‐only vs. the sham and EPC treatment groups (both p < 0.01). Expressions of vWF and eNOS in the dorsal artery were significantly higher in the EPC treatment than the vehicle‐only group ( p < 0.05). In conclusion, EPC treatment restored erectile function in a rat model of bilateral CN injury through recruitment of EPC s toward the dorsal artery and preservation of smooth muscle cells in the corpus cavernosum. These findings elucidate the therapeutic potential of EPC s for treating ED in humans.