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Correlation between sperm DNA fragmentation index and CMA 3 positive spermatozoa in globozoospermic patients
Author(s) -
Hosseinifar H.,
Yazdanikhah S.,
Modarresi T.,
Totonchi M.,
Sadighi Gilani M. A.,
Sabbaghian M.
Publication year - 2015
Publication title -
andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 43
eISSN - 2047-2927
pISSN - 2047-2919
DOI - 10.1111/andr.12030
Subject(s) - sperm , dna fragmentation , andrology , chromomycin a3 , semen , acrosome , biology , sperm motility , staining , positive correlation , semen analysis , population , chromatin , dna , genetics , infertility , apoptosis , medicine , pregnancy , programmed cell death , heterochromatin , environmental health
Summary The absence of the acrosome causes the situation which is called globozoospermia. There are a few studies, mostly as case reports, about correlation between levels of sperm DNA damage in patients with total round‐headed spermatozoa. We investigated this correlation as well as CMA 3 positive spermatozoa in 20 globozoospermic men (with more than 90% round‐headed spermatozoa) attending to Royan Institute. Semen samples divided into three parts to semen analysis, to measure DNA fragmentation index ( DFI ) using sperm chromatin structure assay ( SCSA ) and to detect CMA 3 + sperm cells by chromomycin A3 staining and fluorescent microscopy. Our results showed that there were significant differences in sperm concentration, total sperm motility, and normal morphology between patients and controls group ( p < 0.001). Moreover, the average of DFI and CMA 3 positive spermatozoa in patients group significantly increases compared with control group ( p < 0.001). A significant correlation between DFI and CMA 3 + in total population was also detected in patients group ( r = 0.45, p = 0.046). To our knowledge, this is the largest study about correlation between DNA damage levels and CMA 3 positive spermatozoa with round head sperm cells in total globozoospermic men. It seems that the increase in DNA damage may be because of defective sperm DNA compaction, as we detected CMA 3 positive sperm cells in these patients.