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Modified MYOMI‐14 Korean herbal formulations have protective effects against cyclophosphamide‐induced male infertility in mice
Author(s) -
Park Arum,
Yang Yunjeong,
Jo Junyoung,
Yoon Suk Ran
Publication year - 2021
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.14025
Subject(s) - cyclophosphamide , infertility , spermatogenesis , male infertility , sperm , andrology , sperm motility , motility , fertility , oxidative stress , semen , creb , medicine , endocrinology , biology , chemotherapy , gene , biochemistry , genetics , pregnancy , population , environmental health , transcription factor
Korean herbal formulation, MYOMI‐14, has been reported to improve the idiopathic male infertility condition with poor semen. In this study, four MYOMI formulations were modified from MYOMI‐14 by reducing the number of constituents. We investigated the therapeutic effect of MYOMI formulations on cyclophosphamide‐induced male infertility using mice model. Cyclophosphamide treatment significantly decreased body weight, testicular weight, sperm count, normal‐shaped sperm rate and sperm motility compared to untreated control group, whereas MYOMI formulations restore the cyclophosphamide‐induced dysfunction, as determined by increased sperm count and motility, and decreased abnormally shaped spermatozoa. In addition, treatment with MYOMI formulations reduced cyclophosphamide‐induced apoptosis of germ cells and oxidative stress. MYOMI‐treated mice also showed improved spermatogenesis as shown by the increased expression of spermatogenesis‐related genes, as cAMP‐responsive element modulator (CREM) and cAMP response element‐binding (CREB) protein. Among the MYOMI formulations, MYOMI‐7 showed better results in terms of recovering CP‐induced damages to testis and improving the fertility. Taken together, this study is expected to make significant contribution to the literature by exploring various formulations that reduced constituents of MYOMI‐14, a Korean herbal medicine, in treating CP‐induced male infertility.