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Redox potential in human semen: Validation and qualification of the MiOX sys assay
Author(s) -
Vassiliou Anna,
Martin Catherine H.,
Homa Sheryl T.,
Stone Jesse,
Dawkins Andrew,
Genkova Mariya N.,
Skyla Dela Roca Helen,
Parikh Sejal,
Patel Jinal,
Yap Tet,
Killeen Aideen P.
Publication year - 2021
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13938
Subject(s) - chemiluminescence , semen , redox , male infertility , chemistry , oxidative stress , semen analysis , sperm , andrology , infertility , medicine , chromatography , biology , biochemistry , organic chemistry , pregnancy , genetics
Seminal oxidative stress (OS) is a major contributing factor to male infertility. Semen analysis cannot identify reactive oxygen species (ROS), which can be measured using a chemiluminescence assay. Measurement of redox potential provides a more comprehensive assessment of OS, although the test has yet to be fully validated. This study aimed to validate the MiOX sys analyser for measuring static oxidation–reduction potential (sORP). Results demonstrated that duplicate measurements must be taken, sensors must be batch tested, and sockets should be regularly changed to avoid inconsistency in measurement. Measurement of sORP using MiOX sys exhibited good reproducibility across different operators ( p = 0.469), analysers ( p = 0.963) and days ( p = 0.942). It is not affected by mechanical agitation ( p = 0.522) or snap freezing and thawing ( p = 0.823). The stability of sORP over time requires further verification, particularly in samples with high initial sORP. Measurement is temperature sensitive between 2 and 37°C, significantly increasing with increasing temperature ( p = 0.0004). MiOX sys is a more stable assay for assessing OS than chemiluminescence methods and permits greater flexibility for sample handling. MiOX sys could be implemented to complement semen analysis as part of routine diagnostic testing for male infertility and may be useful in identifying contributing factors to idiopathic infertility.