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High HbA 1c levels affect motility parameters and overexpress oxidative stress of human mature spermatozoa
Author(s) -
Chemlal Hanane,
Bensalem Sihem,
Bendiab Keltouma,
Azzar Meriem,
Benberkane Amine,
Lalaoui Kamel,
IguerOuada Mokrane,
Bournine Lamine
Publication year - 2021
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13902
Subject(s) - oxidative stress , sperm , andrology , motility , thiobarbituric acid , lipid peroxidation , semen , endocrinology , medicine , sperm motility , chemistry , diabetes mellitus , biology , microbiology and biotechnology
The aim of this study is to investigate, by a validated in vitro model, the effect of diabetic plasma on ejaculated human spermatozoa. Plasma of 51 male diabetic patients (mean age 62.28 ± 9.28 years) was selected according to their HbA 1c level: low HBA 1c ≤ 5% (31 mmol/mol), moderate HBA 1c 6%–8% (42–64 mmol/mol) and high HBA 1c ≥ 10% (86 mmol/mol). The plasma was tested on eighteen normal semen samples by analysing gametes motility using a computer Sperm Class Analyzer® and their corresponding oxidative stress (OS) status using thiobarbituric acid‐reactive substances assay. The results indicated that diabetic plasma affected all sperm motility parameters with high HbA 1c showing the most important deleterious effects. Low gametes' straight‐line velocity was observed in high HbA 1c level, mainly after 20 min of co‐incubation (8.78 ± 0.47 µm/s). Also, the highest lipid peroxidation (nmoles MDA/10 8 SPZ) was observed in high HbA 1c values (0.92 ± 0.09), higher than those in spermatozoa treated with H 2 O 2 (0.85 ± 0.04). Conclusively, a direct impact of diabetic plasma on spermatozoa is revealed with overexpression of OS as the underlying mechanism. These findings suggested that it is strongly recommended to control clinically the glycaemic level and OS in diabetic patients for the maintenance of male fertility.