Premium
Nanocurcumin versus mesenchymal stem cells in ameliorating the deleterious effects in the cadmium‐induced testicular injury: A crosstalk between oxidative and apoptotic markers
Author(s) -
Abdel Latif Hany,
Abdel Khalek Rasha,
AbdelGalil Walid,
AbdAllah Hend,
Fawzy Ahmad,
AbdelFattah Shereen
Publication year - 2020
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13760
Subject(s) - mesenchymal stem cell , malondialdehyde , superoxide dismutase , proliferating cell nuclear antigen , lipid peroxidation , andrology , glutathione , apoptosis , cadmium chloride , chemistry , curcumin , biology , oxidative stress , immunology , endocrinology , cadmium , pharmacology , immunohistochemistry , medicine , biochemistry , microbiology and biotechnology , organic chemistry , enzyme
Cadmium (Cd), a grave occupational pollutant, can result in; testicular damage. This study was designed to distinguish the potential effect of bone marrow‐derived mesenchymal stem cells (BM‐MSCs) versus that of curcumin nanoemulsion on Cd‐induced testicular damage. Fifty adult male Sprague Dawley rats were distributed into five groups; control, sham control, Cd‐treated, stem cell‐treated and nanocurcumin‐treated groups. Histological, immune histochemical; caspase 3 and proliferating cell nuclear antigen (PCNA) and CD 68, testosterone levels, nitric oxide, malondialdehyde (MDA)/glutathione (GSH) superoxide, dismutase (SOD), Western blot; B‐cell lymphoma (Bcl‐2), BCL2‐Associated X Protein (BAX), BAX/Bcl‐2 ratio and morphometry were done. Cadmium‐treated group showed degenerated, detached seminiferous tubules, vacuolations and wide interstitial spaces containing fluid exudates. The same group revealed increased expression of BAX, BAX/Bcl‐2 ratio, caspase 3, CD 68 and increased mean values of MDA, NO. Concomitantly, Cd has significant reduction in PCNA, Bcl‐2 and sperm cell count when compared to control group. BM‐MSCs‐ and nanocurcumin‐treated groups revealed well‐structured tubules and were perceived to expressively enhance the deleterious changes induced by Cd. The injurious changes on the testis induced by Cd were obviously improved when treated with either MSCs or nano‐curcumin. BM‐MSCs exerted more ameliorative changes.