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Alteration of sperm parameters and reproductive hormones in Swiss mice via oxidative stress after co‐exposure to titanium dioxide and zinc oxide nanoparticles
Author(s) -
Ogunsuyi Opeoluwa M.,
Ogunsuyi Olusegun I.,
Akanni Olubukola,
Alabi Okunola A.,
Alimba Chibuisi G.,
Adaramoye Oluwatosin A.,
Cambier Sebastien,
Eswara Santhana,
Gutleb Arno C.,
Bakare Adekunle A.
Publication year - 2020
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13758
Subject(s) - oxidative stress , sperm , chemistry , reproductive toxicity , sperm motility , zinc , toxicity , titanium dioxide , endocrinology , andrology , medicine , biochemistry , materials science , organic chemistry , metallurgy
In this study, Swiss male mice were intraperitoneally administered with titanium dioxide (TiO 2 ) and zinc oxide (ZnO) nanoparticles (NPs) and their mixture (1:1) at doses between 9.38 and 75 mg/kg for 5 weeks to evaluate reproductive toxicity. Both NPs and their mixture significantly ( p < .001) altered sperm motility, reduced sperm numbers and increased abnormalities, while their mixture induced more sperm abnormalities than either TiO 2 NPs or ZnO NPs. Both NPs and their mixture significantly ( p < .05) reduced the LH level, while ZnO NPs alone and their mixture ( p < .001) increased the testosterone levels at tested doses. The testes of exposed mice showed pathological changes and altered histomorphometrics. TiO 2 NPs and ZnO NPs individually induced a significant ( p < .01) reduction in SOD and CAT activities, while the mixture significantly ( p < .001) decreased CAT activity and increased SOD activity. TiO 2 NPs alone at 9.38 mg/kg induced a significant ( p < .001) reduction in the GSH level, while both NPs and their mixture increased the MDA level significantly ( p < .05). The data showed that the mixture had a synergistic interaction to induce testicular damage. Overall, oxidative stress may be involved in the NP‐mediated testicular damage observed.