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Low androgen status inhibits erectile function by up‐regulating the expression of P2X receptors in rat corpus cavernosum
Author(s) -
Guo Chuan,
Jiang Jun,
Cheng Bo,
Xie Libo,
Lin Haocheng,
Jiang Rui
Publication year - 2020
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13627
Subject(s) - enos , castration , medicine , endocrinology , androgen , androgen receptor , testosterone (patch) , erectile tissue , rock2 , receptor , rhoa , nitric oxide , chemistry , erectile dysfunction , nitric oxide synthase , signal transduction , hormone , prostate cancer , cancer , biochemistry
The aim of our study was to investigate whether low androgen level inhibits the erectile function of rats by regulating the expression of P2X receptors. Thirty‐six 8‐week‐old male SD rats were randomly divided into six groups: sham‐operated groups (4w‐sham, 8w‐sham), castration groups (4w‐cast, 8w‐cast) and androgen replacement after castration groups (4w‐cast + T, 8w‐cast + T). The maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP), the levels of serum testosterone (T) and nitric oxide (NO), and the expression of P2X1, P2X2, P2X3, eNOS, p‐eNOS, ROCK1 and ROCK2 in the cavernous tissue of rats were determined. The serum T, ICPmax/MAP and NO levels in penile corpus cavernosum in the castration groups were significantly lower than those in other groups ( p < .01). The protein expression of P2X1, P2X2, P2X3, ROCK1 and ROCK2 in the castration groups was significantly higher than those in other groups ( p < .01). P‐eNOS/eNOS of the castration groups were significantly lower than those of other groups ( p < .01). The serum T level was negatively correlated with the expression of P2X1, P2X2 and P2X3 in the corpus cavernosum. Low androgen level inhibits erectile function by up‐regulating the expression of P2X1, P2X2, P2X3 and RhoA/Rho‐kinase resulting in reducing the ratio of p‐eNOS/eNOS and the level of NO in corpus cavernosum of rats.