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Incubation of frozen‐thawed llama sperm with seminal plasma
Author(s) -
Fumuso Fernanda Gabriela,
Giuliano Susana Marí,
Chaves Graciela,
Neild Deborah M.,
Miragaya Marcelo Horacio,
Bertuzzi Mariana LucÍa,
Carretero Maria Ignacia
Publication year - 2020
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13597
Subject(s) - semen , sperm , andrology , incubation , cryopreservation , sperm motility , biology , motility , semen analysis , medicine , embryo , biochemistry , infertility , genetics , pregnancy
Seminal plasma is intimately connected to sperm physiology and particularly in South American Camelids, has demonstrated to be involved in multiple physiological reproductive events. Different percentages of seminal plasma (0%, 10% and 50%) were added to thawed llama semen samples with the objective of evaluating the interaction with cryopreserved sperm over time (0, 1.5 and 3 hr at 37°C). A total of 20 ejaculates from five adult llama males ( n = 5; r = 4 ) were evaluated. A significant decrease in sperm motility, membrane function and live sperm was observed in all thawed samples (0%, 10% and 50%) at 0 hr when compared to raw semen. Neither morphology nor chromatin condensation was altered in all thawed samples ( p > .05), but a significant increase in the percentage of spermatozoa with fragmented DNA was observed after thawing all samples versus raw semen. When evaluating thawed samples over time, a significant decrease of motility and membrane function was observed, while the percentages of total live sperm were preserved over the 3 hr of incubation in all final concentrations evaluated. To conclude, the addition of 10% or 50% of seminal plasma was incapable of preserving motility or membrane function of frozen‐thawed llama sperm during 3 hr of incubation.