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MicroRNA‐based regulatory circuit involved in sperm infertility
Author(s) -
Rahbar Sara,
Pashaiasl Maryam,
Ezzati Maryam,
Ahmadi AsrBadr Yadollah,
MohammadiDehcheshmeh Manijeh,
Mohammadi Seyed Abolgasem,
Ghaffari Novin Marefat
Publication year - 2020
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13453
Subject(s) - azoospermia , microrna , male infertility , spermatogenesis , sperm , andrology , biology , infertility , obstructive azoospermia , real time polymerase chain reaction , regulation of gene expression , gene , medicine , genetics , pregnancy
miRNAs (MicroRNAs), known as noncoding and important endogenous factors regulating the expression protein‐coding genes, are vital regulators in each biological process. Thus, this study aims to explore the key role of four microRNAs in regulating the spermatogenesis. To conduct this experiment, 55 infertile and fertile men provided the study with the sperm and testicular tissue samples. To study the spermatozoa in terms of the morphology, Diff‐Quick was applied. Then, quantitative real‐time polymerase chain reaction (RT‐PCR) was conducted on samples. Our data indicated that in contrast to the miR‐15b, significant increasing of miR‐383 and miR‐122 occurred in both severe oligoasthenoteratozoospermia (SOAT) and moderate oligoasthenoteratozoospermia (MOAT) compared to normal sperm group (N). In addition, it was observed that miR‐15b and miR‐122 increased in patients with nonobstructive azoospermia (NOA) compared with obstructive azoospermia (OA) group. Expression levels of target genes including P53 , CASPASE‐9 and CYCLIN D1 underwent principle changes according to miRNAs expression level. Our finding indicated that miRNAs had essential role in the regulation of spermatogenesis, and their expression altering was associated with sperm abnormalities. Thus, microRNAs can be introduced as useful biomarkers to determine male infertility reasons to choose the effective treatment.