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Addition of pomegranate juice ( Punica granatum ) in tris‐based extender improves post‐thaw quality, motion dynamics and in vivo fertility of Nili Ravi buffalo ( Bubalus bubalis ) bull spermatozoa
Author(s) -
Javed Moazam,
Tunio Muhammad Tarique,
Abdul Rauf Hafiz,
Bhutta Muhammad Fahad,
Naz Sadia,
Iqbal Sajid
Publication year - 2019
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13322
Subject(s) - extender , bubalus , punica , semen , sperm , acrosome , zoology , biology , andrology , sperm motility , semen analysis , semen quality , chemistry , food science , anatomy , botany , medicine , infertility , horticulture , genetics , ecology , organic chemistry , polyurethane , pregnancy
The aim of the present study was to determine the protective effects of pomegranate juice in tris‐based extender on semen parameters, computer‐assisted sperm analysis (CASA) motion characteristics and field fertility of post‐thawed Nili Ravi buffalo ( Bubalus bubalis ) bull spermatozoa. Two consecutive ejaculates/collection from each of the five adult Nili Ravi buffalo bulls were collected with artificial vagina at 42°C for a period of 7 weeks, diluted in extender containing different concentrations of pomegranate juice (0.0%, 2.5%, 5%, 7.5% and 10%). Diluted samples were packed and frozen in 0.54 ml French straws. The addition of 10% pomegranate juice in extender significantly improved post‐thaw sperm morphology (%), motilities (CASA total motility, progressive motility (%) as well as VAP, VSL, VCL, STR, DAP, DSL) compared to the control group ( p < 0.05). Plasma membrane, acrosome membrane and DNA integrity were significantly higher in extender with 10% pomegranate juice than the control group ( p < 0.05). Field fertility rate (60.39% vs. 46.53%) was higher ( p < 0.05) in extender with 10% pomegranate juice as compared to the control. It is therefore concluded that the addition of 10% pomegranate juice in tris‐based extender improves post‐thaw semen parameters, CASA motion dynamics and field fertility in Nili Ravi buffaloes.