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Oxidative stress‐induced alterations in seminal plasma antioxidants: Is there any association with keap1 gene methylation in human spermatozoa?
Author(s) -
Darbandi Mahsa,
Darbandi Sara,
Agarwal Ashok,
Baskaran Saradha,
Sengupta Pallav,
Dutta Sulagna,
Mokarram Pooneh,
Saliminejad Kioomars,
Sadeghi Mohammad Reza
Publication year - 2019
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13159
Subject(s) - oxidative stress , keap1 , methylation , reactive oxygen species , andrology , epigenetics , dna methylation , semen , biology , antioxidant , sperm , medicine , endocrinology , genetics , gene , biochemistry , gene expression , transcription factor
Kelch‐like ECH‐associated protein 1 ( keap1 )‐nuclear factor‐erythroid 2‐related factor 2 (Nrf2) pathway is one of the master regulators of cellular defence against oxidative stress. Epigenetic alterations like hypermethylation of keap1 gene impair keap1 ‐Nrf2 system in several oxidative stress–associated diseases. The objective of this study was to evaluate the epigenetic status of keap1 in sperm DNA of normozoospermic subjects, having different levels of reactive oxygen species (ROS) in seminal plasma. Semen samples were obtained from 151 apparently healthy male partners of couples who attended the Avicenna infertility clinic. Samples were categorised into four groups according to their ROS levels: group A ( n = 39, ROS < 20 RLU/s per 10 6 spermatozoa), group B ( n = 38, 20 ≤ ROS < 40 RLU/s per 10 6 spermatozoa), group C ( n = 31, 40 ≤ ROS < 60 RLU/s per 10 6 spermatozoa) and group D; ( n = 43, ROS ≥ 60 RLU/s per 10 6 spermatozoa). Keap1 methylation status was assessed using methylation‐specific PCR along with seminal total antioxidant capacity. The results showed no significant alterations in keap1 methylation in any groups, whereas the total antioxidant capacity enhanced with increasing levels of ROS exposure. These results indicate that keap1 was not methylated during ROS elevation and oxidative stress, suggesting that the cells have adopted other mechanisms to elevate antioxidant level.