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Prognostic value of thiol/disulphide homeostasis in predicting testicular ischaemia–reperfusion injury in rats
Author(s) -
Urkmez Ahmet,
Kutluhan Musab A.,
Topaktas Ramazan,
Gumrukcu Gulistan,
Erel Ozcan,
Ozturk Metin I.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13134
Subject(s) - testicular torsion , ischemia , medicine , thiol , homeostasis , reperfusion injury , endocrinology , urology , surgery , chemistry , biochemistry
Testicular torsion is a surgical emergency and the testicular salvage rate through surgical detorsion ranges from 42% to 88%. However, it is not known to what extent spermatogenic function is preserved in these testes. The purpose of the study was to demonstrate the prognostic value of thiol/disulphide homeostasis in rats for testicular ischaemia and ischaemia–reperfusion injury during early and late stages. A total of 21 Wistar albino rats were randomly divided into three groups. The 4 and 8‐hr T/D (torsion/detorsion) groups were subjected to left testicular torsion by twisting the testes by 720° counterclockwise direction. The 2 cc venous blood samples were also collected from the 4 and 8‐hr T/D groups after 4 and 8 hr respectively. It was determined that the native thiol, total thiol and disulphide values of 4 and 8‐hr T/D group before detorsion were significantly lower than those of the sham group ( p : 0.006; p : 0.003; p : 0.003). In the 8‐hr T/D group, there was a positive statistically significant relationship at an 88.3% level between Johnsen's score and total thiol values before detorsion ( p : 0.008). Our study showed that thiol/disulphide homeostasis may be a haematologic parameter in predicting testicular ischaemia and histopathologic injury in the testes following ischaemia–reperfusion.