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Positive effect of progesterone on motility and velocity of fresh, vitrified without permeable cryoprotectants and frozen with permeable cryoprotectants human spermatozoa
Author(s) -
Saymé Nabil,
Dite Lisa,
Krebs Thomas,
Kljajić Marija,
Maas Dieter H. A.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13133
Subject(s) - cryoprotectant , cryopreservation , motility , andrology , sperm , capacitation , semen , sperm motility , incubation , vitrification , biology , chemistry , medicine , embryo , biochemistry , microbiology and biotechnology
Steroid hormone progesterone has been found to play an important role in the migration of spermatozoa through the reproductive tract, as well as to induce hyperactive motility and increase sperm velocity. The aim of this study was to examine whether progesterone could induce beneficial effects in vitrified and slow‐frozen spermatozoa. During the research process, 50 semen samples were divided into three treatment groups; noncryopreserved, slow‐freezing and vitrification. After thawing and an incubation period of 2 hr to induce capacitation, semen samples from each treatment group were treated with 50 nM, 25 nM progesterone and a control solution for 30 min. Thereafter, the sperm suspensions were examined manually to assess the proportion of viable and motile spermatozoa, as well as using the CASA to evaluate the velocity parameters. The results indicated a higher proportion of progressively motile spermatozoa in vitrified teratozoospermic samples and improved velocity parameters in slow‐frozen normozoospermic and teratozoospermic samples. The main conclusion of this research was that the used progesterone concentration of 50 nM was sufficient to significantly improve the motility of vitrified teratozoospermic samples and velocity parameters of cryopreserved sperm samples. The present findings might have important implications in determining ways of improving the current low rates of motility in cryopreserved spermatozoa.