Premium
Changes in sperm function and structure after freezing in domestic cat spermatozoa
Author(s) -
Cheuquemán Carolina,
Faúndez Ricardo,
Sánchez Raul,
Risopatrón Jennie
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13080
Subject(s) - sperm , andrology , cryopreservation , acrosome , semen , sperm motility , motility , oxidative stress , semen cryopreservation , biology , chemistry , microbiology and biotechnology , biochemistry , medicine , embryo
Sperm cryopreservation allows for a long‐term storage of genetic. However, changes due to factors as cold shock, osmotic and oxidative stress cause reduction in viability and fertilising ability of frozen/thawed spermatozoa. Therefore, evaluation of cryoinjury of cat spermatozoa is a key factor in achieving better cryopreservation results. This study analysed the changes in structural and functional after freezing in ejaculated domestic cats spermatozoa. Semen samples ( n = 60) were analysed before and after freezing, progressive motility was determined with computer‐assisted sperm analysis and viability, and acrosome intact spermatozoa, mitochondrial function and superoxide anion ( O 2 - ) were assessed by flow cytometry. The results demonstrated that cryopreservation induced changes in all sperm parameters ( p < 0.05). Total sperm motility, viability, acrosome integrity and mitochondrial function of fresh samples were near to 80% and decrease near to 40% in frozen/thawed spermatozoa ( p < 0.05); nevertheless, in contrast to all other sperm parameters, the sperm positive with O 2 - increased post/thawing ( p < 0.05). In conclusion, changes in frozen/thawed spermatozoa could be related to the effect of oxidative stress due to the increase in the synthesis of O 2 - and a concomitant loss of functional competence. Therefore, the evaluation of these sperm parameters could contribute to complement the analysis of fresh or frozen semen used ART.