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Comparison of Tris egg yolk‐based, Triladyl ® and Optixell ® extender on post‐thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo (Bubalus bubalis) bull spermatozoa
Author(s) -
Naz Sadia,
Umair Muhammad,
Iqbal Sajid
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.13063
Subject(s) - extender , semen quality , semen , semen cryopreservation , bubalus , biology , andrology , sperm , acrosome , yolk , semen extender , anatomy , sperm motility , chemistry , medicine , food science , ecology , organic chemistry , polyurethane
Our objectives were to ascertain the comparison of Tris egg yolk‐based, Triladyl ® and Optixell ® extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo ( Bubalus bubalis ) bull spermatozoa. Semen samples ( n = 35) from five bulls were diluted in Tris egg yolk‐based, Triladyl ® , Optixell ® extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher ( p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk‐based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross‐frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher ( p < 0.05) in spermatozoa cryopreserved in Optixell ® extender as compared to Tris egg yolk‐based and Triladyl ® extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher ( p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk‐based and Triladyl ® extender respectively. It is concluded that Optixell ® extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.