Comparison of Tris egg yolk‐based, Triladyl ®  and Optixell ®  extender on post‐thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo  (Bubalus bubalis)  bull spermatozoa | Zendy

Naz Sadia | Zendy; Umair Muhammad | Zendy; Iqbal Sajid | Zendy

Premium

Comparison of Tris egg yolk‐based, Triladyl ® and Optixell ® extender on post‐thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo (Bubalus bubalis) bull spermatozoa

Author(s) -

Naz Sadia,

Umair Muhammad,

Iqbal Sajid

Publication year - 2018

Publication title -

andrologia

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.633

H-Index - 59

eISSN - 1439-0272

pISSN - 0303-4569

DOI - 10.1111/and.13063

Subject(s) - extender , semen quality , semen , semen cryopreservation , bubalus , biology , andrology , sperm , acrosome , yolk , semen extender , anatomy , sperm motility , chemistry , medicine , food science , ecology , organic chemistry , polyurethane

Our objectives were to ascertain the comparison of Tris egg yolk‐based, Triladyl ® and Optixell ® extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo ( Bubalus bubalis ) bull spermatozoa. Semen samples ( n = 35) from five bulls were diluted in Tris egg yolk‐based, Triladyl ® , Optixell ® extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher ( p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk‐based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross‐frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher ( p < 0.05) in spermatozoa cryopreserved in Optixell ® extender as compared to Tris egg yolk‐based and Triladyl ® extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher ( p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk‐based and Triladyl ® extender respectively. It is concluded that Optixell ® extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research