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Identification of reference genes and mi RNA s for RT ‐ qPCR in testosterone propionate‐induced benign prostatic hyperplasia in rats
Author(s) -
Chen X.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12966
Subject(s) - hyperplasia , testosterone propionate , reference genes , gene expression , real time polymerase chain reaction , microrna , gene , prostate , testosterone (patch) , biology , medicine , andrology , endocrinology , androgen , cancer , genetics , hormone
Summary Testosterone propionate‐induced benign prostatic hyperplasia in rats is a common model that is widely used in studies of the effects and molecular mechanisms of drugs designed to treat benign prostatic hyperplasia. RT ‐ qPCR is a widely used technique in gene expression studies. Proper normalisation is critical for accurate expression analysis. Currently, no validated reference genes are available for RT ‐ qPCR in rat benign prostatic hyperplasia. Given that micro RNA s regulate mRNA expression at the post‐transcriptional level, they are usually studied together. Here, the expression stability of 21 putative reference genes including 8 mRNA s and 13 mi RNA s was evaluated in benign prostatic hyperplasia in rats. Relative expression levels of each gene were detected in rats from a model group and a normal group using SYBR RT ‐ qPCR . Expression stability was evaluated by geNorm and NormFinder. The commonly used reference genes, such as ACTB , B2M and mir‐16, were less stable, and let‐7a was eliminated due to a large C t value, most likely indicating a relatively low expression level. Therefore, to obtain reliable results, mir‐26a was recommended as a suitable reference for mi RNA expression analysis and EF ‐1a as a suitable reference for mRNA analysis in testosterone propionate‐induced benign prostatic hyperplasia in rats.