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Human sperm handling in intracytoplasmic sperm injection processes: In vitro studies on mouse oocyte activation, embryo development competence and sperm oxidation–reduction potential
Author(s) -
Roychoudhury S.,
MaldonadoRosas I.,
Agarwal A.,
Esteves S. C.,
Henkel R.,
Sharma R.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12943
Subject(s) - intracytoplasmic sperm injection , oocyte , oocyte activation , sperm , andrology , embryo , embryogenesis , human fertilization , chemistry , in vitro fertilisation , biology , microbiology and biotechnology , anatomy , medicine
Summary Polyvinylpyrrolidone ( PVP ) and hyaluronic acid ( HA ) are routinely used in handling spermatozoa for intracytoplasmic sperm injection ( ICSI ). As there are still concerns about possible adverse effects on the embryo, this study investigated sperm handling in a mouse ICSI model to (i) evaluate oocyte activation after injection of spermatozoa selected for rotational or linear motion in PVP ; (ii) assess the effect of sperm selection in PVP , HA and medium on oocyte activation; (iii) examine the effects of PVP and HA on parthenogenetic oocyte activation and embryo development; and (iv) assess the oxidation–reduction potential ( ORP ) of spermatozoa exposed to PVP , HA or medium. Oocyte activation was higher when spermatozoa exhibited rotational motion rather than linear motion (79% vs. 52%; p = .05). There was no difference in oocyte activation and embryo development after parthenogenetic oocyte activation after sperm injection using PVP , HA or medium‐incubated spermatozoa. PVP ‐selected spermatozoa exhibited lower ( p < .0001) ORP levels than using HA . Thus, results indicate that the sperm handling method and the type of medium used impact ICSI outcomes. Overall, sperm incubation in PVP , HA and medium yields similar outcomes with regard to oocyte activation and embryo development. However, PVP provides more antioxidative protection than HA and should therefore be preferred for sperm manipulation.