Premium
Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls
Author(s) -
Ferreira C. E. R.,
Haas C. S.,
Goularte K. L.,
Rovani M. T.,
Cardoso F. F.,
Schneider A.,
Gasperin B. G.,
Lucia T.
Publication year - 2018
Publication title -
andrologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.633
H-Index - 59
eISSN - 1439-0272
pISSN - 0303-4569
DOI - 10.1111/and.12923
Subject(s) - epididymis , paraoxonase , pon1 , andrology , biology , sperm , semen , medicine , motility , endocrinology , sperm motility , oxidative stress , biochemistry , gene , genetics , genotype
Summary The paraoxonases types 1, 2 and 3 ( PON 1, PON 2 and PON 3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON 1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON 1 , PON 2 and PON 3 was characterised by RT ‐ PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON 2 and PON 3 were both expressed in the epididymis head and PON 3 was also expressed in the epididymis tail. The PON 1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation ( r = .90) between the activity of the PON 1 in both serum and seminal plasma ( p < .0001). The PON 1 activity in the seminal plasma was positively correlated with ejaculate's colour, sperm mass activity ( p = .04), motility, vigour and viability (all p < .01). Thus, PON 1 may be a potential marker for sperm motility and viability in bulls.